Intracytoplasmic Sperm Injection (ICSI) Fertility Treatment

Intracytoplasmic Sperm Injection (ICSI) – IVF
Intracytoplasmic Sperm Injection (ICSI) is a specialised form of In Vitro Fertilisation (IVF) that is used for the treatment of severe cases of male-factor infertility. ICSI involves the injection of a single sperm directly into a mature egg.At City Fertility Centre, we use a medium named Sperm Slow™ during sperm selection.
This medium contains hyaluronan (HA), which binds sperm that are more likely to have normal DNA, and thus allows selection of these bound sperm for injection. By selecting the sperm that are bound to HA and using them for ICSI, the embryologists are preferentially using the better-quality, more mature sperm. This technique is also known as PICSI and we use it as standard practice with no additional cost to the patient.
Before ICSI can be carried out, mature eggs must be retrieved from the female partner during a standard IVF cycle. The male partner’s semen sample is prepared in the lab to isolate as many healthy, moving sperm as possible. After allowing the eggs to rest for two to three hours following their removal, the tight outer coating of cells (cumulus) is removed from each egg. Only then can we be sure the egg is mature enough to undergo ICSI.
Immature eggs cannot be injected. However, they can be incubated for a further two to six hours and reassessed. If they mature in that time, they can still be injected along with the other mature eggs.
A special instrument is used to hold the egg in place. It is so small you can barely see the tip with the naked eye. A thinner, sharp, needle-like instrument is used to pick up a single sperm.
Dr Abha Majumdar is best IVF Expert in Delhi. Dr. Abha Majumdar is a Gynecologist in New Rajendra Nagar, Delhi and has an experience of 41 years in this field. Dr Abha Majumdar practices at Genesis Clinic in New Rajendra Nagar, Delhi and Sir Ganga Ram Hospital in Old Rajendra Nagar, Delhi. She completed MBBS from University Of Agra in 1976 and MS – Obstetrics & Gynaecology from University Of Agra in 1978.She is a member of Indian Society for Assisted Reproduction (ISAR), Indian Fertility Society (IFS), National Association for Reproductive & Child Health of India (NARCHI), Federation of Obstetric and Gynaecological Societies of India (FOGSI) and European Society of Human Reproduction and Embryology (ESHRE).

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